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MOSHAV BNEI ZION
POST OFFICE BOX 76
CODE 60910, ISRAEL

TEL (+) 972-9-903020
FAX (+) 972-9-903022

OUR REF 96012841.A

DATE 28-1-96

6600 - 1 DAY OLD TURKEY CHICKS WERE HOUSED IN 500M2 PEN.
THEN WAS DIVIDED BY FENCE - TO TWO SIDES - 250M2 EACH.

GROUP 1 - 3200 MIXED MALE AND FEMALE CHICKS ON 2502 RECEVED 50G/1M2 STLOSAN F POWDER, ONCE A WEEK, ON WOOD CHIPS BEDDING - STARTED ONE DAY BEFORE HOUSING THE CHIKS.

GROUP 2 - CONTROL 3400 MALE CHICKS ON 2502 ON WOOD CHIPS BEDDING.

THE TRIAL WAS TAKEN PLACE IN MOTI SAAD FARM AT MOSHAV OROT - ISRAEL, DURING THE SUMMER 25/7/95 - 22/8/95.

THE RESULTS ARE PRESENTED IN THE TABLES BELOW.

TOWARD THE END OF THE TRIAL THERE WERE CLEAR DIFFERENT BETWEEN BOTH SIDES FOR ANY ONE VISITING THE PEN

TREATMENT WITH STALOSAN F SHOWED:

     1) DECREAS MORTALITY BY 30%
     2) SAVED 26 WOOD CHIPS BAGS.
     3) IMPROVED BEDDING QUALITY.
     4) ELIMINATE AMONIA SMELL.

THE TRIAL TOOK PLACE IN THE SUMMER WITH RELATIVELY HIGH TEMPERATURE THAT ALLOW GOOD VENTILATION OF THE PEN WHICH HELP TO-
     1) DRYING THE BEDDING IN BOTH SIDES.
     2) ELIMINATE AMONIA SMELL.
     3) DECREASE MORBIDITY AND MORTALITY.

IT SEEMS THAT DURING THE WINTER WITH LOW AMBIENT TEMPERATURE WHICH PREVENT POSSIBILITY OF VENTILATION THE DIFFERENCES BETWEEN TREATED AND UNTREATED GROUPS WILL BE GRATER.


AMIR BIRNBAUM - D.V.M

Diagrams show the results of trials
with stalosan F

Following diagrams shows the results af trials with Stalosan F in broiler farms carried out by Dr. Imran at Raiwind Road, Lahore and Dr. Awais Chang-Mnaga site broilers farm, Pakistan. The trials were conducted in October 2001.

Trials were made to check the actual efficacy of Stalosan F. Indeed the farmers liked to use Stalosan F afterwards.

Best regards

1.

Note: FCR was not calculated during first three weeks separately, even then trial results of
Stalosan-F are achieved.

2.

Note: FCR was not calculated during first three weeks separately, even then trial results Of
Stalosan-F are achieved.

3.

Note: FCR was not calculated during first three weeks separately, even then trial results Of Stalosan-F are achieved.

4.

Note: After fifth week, Farmer mixed both groups.
Weight Gain & FCR cold not be calculated.

5.

Note: After fifth week, Farmer mixed both groups.
Weight Gain & FCR cold not be calculated.

16. June 2000

Re: Evaluation of the effect of Stalosan F on poultry endoparasites

Production losses are significantly higher in organic table egg production systems compared to conventional intensive indoor production systems. No methodical analysis of the cause of the higher mortality and morbidity in the organic production of poultry is available at present. However, investigations have shown that infections with intestinal roundworms such as Ascaridia galli, Heterakis gallinarum and Capillaria obsignata are highly prevalent in organic production systems. Infections with such endoparasites have been estimated to cause production losses in the rage of 10-20% due to impaired feed conversion, reduced growth and egg production, and increased mortality. H. gallinarum is of additional importance due to its ability to transfer the protozoa Histomonas meleagridis, which causes Blackhead in poultry. Furthermore, a recently completed study at our department showed that eggs from A. galli can transfer Salmonella enterica to chickens. Additionally, ongoing investigations suggest that endoparasites have a direct impact on the behaviour of chickens, which may induce increased cannibalism.

The potentially high level of disease in the organic production of broilers and eggs for consumption, together with anthelmintic regulations in force, show a marked need for alternative methods of parasite control in organic poultry production.

The undersigned have examined whether Stalosan F could be an alternative or supplement in the control of endoparasites in poultry. Our intention was to investigate the effect of Stalosan F on three commonly occurring endoparasites in poultry, i.e. A. galli, H. gallinarum and C. obsignata, under laboratory conditions and in the field. These investigations have recently been completed and the final report is in preparation. In short, the results suggest that Stalosan F does have an effect on these parasites. In one experiment, a house heavily contaminated with A. galli eggs was parted in two pens; Stalosan F was applied to one pen, whereas the other pen was left as untreated control. Twenty-five day old chicks were introduced to each pen, and, seven weeks later, slaughtered and examined for the presence of A. galli. The chickens from the Stalosan F treated pen, were found to harbour significantly fewer adult worms than the chickens from the untreated pen. However, no differences were found between the two groups in the total number of worms (larvae and adults) recovered. It therefore seems that the development from larvae to adult worm was arrested by the use of Stalosan F. This could be of importance to the epidemiology of the worms, since it would decrease the reproduction rate of the worms hereby reducing the number of parasite eggs in the pen.

Another experiment, where eggs from A. galli, H. gallinarum and C. obsignata were exposed to Stalosan F in the laboratory and afterwards administered to 4 week old chickens, showed that the infectivity of the eggs were significantly reduced as compared to non-treated eggs. This suggests that Stalosan F may have a sublethal effect on the abovementioned parasite eggs.

Therefore, we believe that regular use of Stalosan F in organic poultry production systems may contribute to the control of parasites in poultry production.